河南11选5

河南11选5新闻动态

色谱柱篇:如何延长色谱柱的利用寿命

来源 : 时间 : 2019-03-0556人浏览

[导读]色谱柱的利用寿命,除了与所分析的样品和流动相及利用频率有关系外,主要的是与日常的委会密切相关。

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河南11选5YI、LIUDONGXIANGDEPHYINGZAILIYONGDEFANWEINEIFANXIANGSEPUZHUYOUYUTIANLIAOZHONGCUNZAISi-CHESi-OJIAN,LIUDONGXIANGCHAOGUOQIPHFANWEIJIANGHUIDAOZHIGUIJIAOJIZHILIUSHIHEJIANHEXIANGTANLIANDUANLIE,SHIZHUXIAOXIAJIANG,LIYONGSHOUMINGBIANDUAN。YOUYULIUDONGXIANGDEPH KONGZHIQIANTUOERDUISEPUZHUZAOCHENGDESUNHAI,TONGCHANGHENNANSHISEPUZHUHUIFU,YINCIBIXURENZHENDUIDAI,YANGEKONGZHILIUDONGXIANGDEPHZHI。

ER、QUCHUYANGPINHELIUDONGXIANGZHONGDEGUTIKELI YANGPINHELIUDONGXIANGZHONGHANYOUDEGUTIKELIWUZHIHUIDUSAISEPUZHUSHAIBAN,SHAIBANBEIDUZHUBUJINHUIYINQIZHUYADESHENGGAO,ERQIEYEHUIYINQIZHUXIAOXIAJIANG,YINWEISHAIBANDEDUSAIHUIYINQIYELIUBUJUN,DAOZHISEPUFENGXINGTUOWEI、BIANKUAN,CONGERSHIZHUXIAOXIAJIANG。YINCI,JIANYILIYONGCHAOCHUNSHUIHESEPUCHUNSHIJI,ZAIFENXIYANGPINQIANDUIYANGPINJINXINGZHENTONGGUOLV,LIUDONGXIANGGUO0.45μmLVMO。

三、利用维护柱或在线过滤器 样品和流动相经过滤后并不能完全消除固体颗粒物质,因为泵的磨损、密封圈和管路的老化也会产生固体颗粒物质,这些固体颗粒被流动相带入色谱柱,堵塞筛板,导致柱压升高、柱效下降。维护柱和在线过滤器上都有筛板,其孔径与色谱柱孔径相同,因此能够阻止固体颗粒物质到达色谱柱,有效防止色谱柱筛板的堵塞。由于柱压升高在分析故障中占很大 比例,因此,除对样品和流动相进行过滤外,建议您在色谱柱进样端加上维护柱或在线过滤器。 假设确认色谱柱柱压升高是由于进样端筛板被堵引起的,可选择以下方式进行补救:

1、先在色谱柱前加上维护柱或在线过滤器,然后用甲醇、水=20/80ml/min反向冲色谱柱180min。

2、先在色谱柱进样端加上维护柱或在线过滤器,然后反向利用。

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